Postdoctoral Research Assistant in Single-Molecule Transcription



Parks Road

The Department of Physics is seeking a Postdoctoral Research Assistant to join the Gene Machines’ group, led by Professor Achilles Kapanidis. The post is available for a fixed-term of 36 months. The group is well known for developing single-molecule fluorescence methods (Holden Nature Methods 2011, 8, 279; Crawford Biophys J 2013, 105, 2439) and applying them to DNA/RNA polymerases (Hohlbein Nature Comm, 2013, 4, 2131; Stracy M PNAS 2015; 112, E4390; Duchi Mol Cell 2016; in press).

The project is funded by the Wellcome Trust and focuses on in vivo gene expression and transcription mechanisms. You will prepare recombinant bacterial strains and perform in vivo single-molecule imaging and super-resolution microscopy of RNA polymerase and transcription proteins to capture the real-time transcription kinetics and the spatial organisation of transcription in living bacteria. You will manage academic and administrative activities, develop ideas for generating research income, collaborate on reports and journal articles, and have the opportunity to teach.

The ideal candidate should possess (or be close to obtaining) a doctorate in biochemistry, biophysics, microbiology or a related field, and experience in DNA biochemistry, protein-DNA interactions, microbiology, modern cloning techniques, and quantitative cell imaging. Experience with bacterial transcription and fluorescence is highly desirable. Experience in particle tracking, single-molecule fluorescence, super-resolution imaging, and advanced image analysis is desirable, but not essential. You should also have a strong publication record, excellent communication skills and able to work effectively within an interdisciplinary group.

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Please direct enquiries to Professor Achilles Kapanidis ([email protected]).

Only applications received before 12.00 midday on Monday 19 September 2016 can be considered. You will be required to upload a statement of research interests, CV, copies of two representative publications and details of three referees as part of your online application.

www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.jobspec?p_id=124988

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